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Registro Completo |
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Biblioteca(s): |
Embrapa Amapá; Embrapa Pecuária Sul; Embrapa Unidades Centrais. |
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Data corrente: |
08/01/1991 |
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Data da última atualização: |
24/10/2014 |
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Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
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Autoria: |
GIRARDI-DEIRO, A. M.; GONÇALVES, J. O. N. |
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Título: |
Determinação do tamanho e número de amostras da vegetação do campo natural em Bagé, RS. |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
Bagé: EMBRAPA-CNPO, 1989. |
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Páginas: |
23 p. |
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Série: |
(EMBRAPA-CNPO. Boletim de pesquisa, 14). |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho foi conduzido no CNPO, EMBRAPA, em uma área de campo natural subdividida em 6 potreiros e submetida a três cargas animais distintas. Teve como objetivo determinar o tamanho e o número de unidades de amostra (quadrados) através das curvas representativas das relações entre úmeros de espécies e área, e entre número de espécie e número quadrados. Na interpretação destas curvas foi utilizado, além da observação do formato da curva, o procedimento da CAIN (1938) e também o incremento médio das espécies. |
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Palavras-Chave: |
Brasil; Região Sudoeste; Rio Grande do Sul. |
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Thesagro: |
Campo; Pastagem Nativa; Tamanho; Vegetação; Vegetação Nativa. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/110561/1/DETERMINACAO-DO-TAMANHO-E-NUMERO-OK.pdf
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Marc: |
LEADER 01212nam a2200241 a 4500
001 1219172
005 2014-10-24
008 1989 bl uuuu u0uu1 u #d
100 1 $aGIRARDI-DEIRO, A. M.
245 $aDeterminação do tamanho e número de amostras da vegetação do campo natural em Bagé, RS.
260 $aBagé: EMBRAPA-CNPO$c1989
300 $a23 p.
490 $a(EMBRAPA-CNPO. Boletim de pesquisa, 14).
520 $aEste trabalho foi conduzido no CNPO, EMBRAPA, em uma área de campo natural subdividida em 6 potreiros e submetida a três cargas animais distintas. Teve como objetivo determinar o tamanho e o número de unidades de amostra (quadrados) através das curvas representativas das relações entre úmeros de espécies e área, e entre número de espécie e número quadrados. Na interpretação destas curvas foi utilizado, além da observação do formato da curva, o procedimento da CAIN (1938) e também o incremento médio das espécies.
650 $aCampo
650 $aPastagem Nativa
650 $aTamanho
650 $aVegetação
650 $aVegetação Nativa
653 $aBrasil
653 $aRegião Sudoeste
653 $aRio Grande do Sul
700 1 $aGONÇALVES, J. O. N.
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Registro original: |
Embrapa Pecuária Sul (CPPSUL) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
22/03/2011 |
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Data da última atualização: |
09/09/2014 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ANDREOTTI, R.; SOARES, M.; CUNHA, R. C.; LEITE, F. P. L. |
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Afiliação: |
RENATO ANDREOTTI E SILVA, CNPGC; UFPEL; UFPEL; UFPEL. |
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Título: |
Heterologous expression in Pichia pastoris of gene BMTI optimized. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS OF PARASITOLOGY, 12., 2010, Melbourne. Conference abstracts. Melbourne: The Australian Society for Parasitology:World Federation of Parasitologists, 2010. |
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Páginas: |
p.149-152 |
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Idioma: |
Inglês
Português |
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Notas: |
ICOPA XII |
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Conteúdo: |
The cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same time was recognized by the anti-histidine and by bovine serum immunized with a synthetic based pepitídeo (BmTI). Thus, a recombinant protein (rBmTI) was
expressed, and was recognized by the anti-histidine and characterized as potential antigenic by bovine serum immunized with the synthetic pepitídeo. MenosThe cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same... Mostrar Tudo |
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Thesagro: |
Carrapato; Sanidade Animal. |
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Thesaurus NAL: |
Rhipicephalus microplus. |
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Categoria do assunto: |
H Saúde e Patologia
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02512naa a2200217 a 4500
001 1883447
005 2014-09-09
008 2010 bl --- 0-- u #d
100 1 $aANDREOTTI, R.
245 $aHeterologous expression in Pichia pastoris of gene BMTI optimized.
260 $c2010
300 $ap.149-152
500 $aICOPA XII
520 $aThe cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same time was recognized by the anti-histidine and by bovine serum immunized with a synthetic based pepitídeo (BmTI). Thus, a recombinant protein (rBmTI) was expressed, and was recognized by the anti-histidine and characterized as potential antigenic by bovine serum immunized with the synthetic pepitídeo.
650 $aRhipicephalus microplus
650 $aCarrapato
650 $aSanidade Animal
700 1 $aSOARES, M.
700 1 $aCUNHA, R. C.
700 1 $aLEITE, F. P. L.
773 $tIn: INTERNATIONAL CONGRESS OF PARASITOLOGY, 12., 2010, Melbourne. Conference abstracts. Melbourne: The Australian Society for Parasitology:World Federation of Parasitologists, 2010.
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Embrapa Gado de Corte (CNPGC) |
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